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NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ
品系货号：005557 ｜ 通用名称：NSG
又名：NOD-scid IL2Rgammanull, NOD-scid IL2Rgnull, NOD scid gamma
摘要
这个品系的小鼠通常被引用为它们的商品名“NSG™”，是免疫缺陷最严重的小鼠品系。NSG小鼠的遗传背景是NOD/ShiLtJ，携带有两个突变，联合免疫缺陷突变（scid）和IL2受体通用gamma链完全敲除突变（IL2rgnull）。Scid突变是DNA修复复合物蛋白Prkdc上的突变，导致小鼠T细胞和B细胞缺失。IL2rgnull导致多个基于IL2rg的细胞因子通路受阻，小鼠因而缺少功能成熟的NK细胞。严重的免疫缺陷使得NSG小鼠可以接受多种人源细胞或组织的移植进而人源化，诸如CD34+的造血干细胞（HSC），外周血单核细胞（PBMC），病人来源的异体移植物（PDC)或者是成人干细胞和组织。NSG小鼠被广泛用于多个研究方向，比如人的免疫系统功能，感染性疾病，糖尿病，癌症以及干细胞生物学等。
重要提示：与其背景品系NOD/ShiLtJ(品系号001976)相似， NSG小鼠表现出进行性脱毛，到3月龄时通常已经很严重。
品系特点
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同源
偶发突变
靶向突变
捐赠者
Leonard Shultz博士——杰克森实验室
原始参考文献
如果在文章中使用了NSG小鼠品系，请引用原始文献并在材料与方法部分注明JAX品系号#005557
查阅参考文献 > 如果在文章中使用了NSG小鼠品系，请引用原始文献并在材料与方法部分注明JAX品系号#005557品系详情
NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ小鼠（通常以其商品名 NOD scid gamma (NSG™) 为人所知）既不表达 Prkdc 基因，也不表达 X 连锁 Il2rg 基因。NSG 小鼠可存活且可繁育、体型正常，无任何可见的身体和行为异常。淋巴组织的组织学检查显示，胸腺中无淋巴样细胞和某些囊性结构，脾脏中无滤泡，淋巴结细胞结构显著减少。NSG 小鼠缺乏成熟淋巴细胞、无法检测到血清 Ig，自然杀伤 (NK) 细胞的细胞毒活性极低。该品系在经过亚致死辐射处理后也不易发生淋巴瘤。NSG 小鼠经证明可支持高效的人 CD34+ 造血干细胞移植，适用于长期稳定的异种移植研究。NSG小鼠对于可致糖尿病的链佐霉素（STZ）高度敏感。
尾部扭曲，头部歪斜，转圈以及后肢无力在NSG品系中的发生率很低，在实验用未经处理的幼鼠中的发生率通常低于0.01%， 在经过人源化或其它研究处理的成年鼠中发生率通常低于0.5%。
JAX® 服务团队还可以提供用于胰岛移植研究的NSG-STZ临床前模型。
需要注意的是，NSG品系中IL-2受体gamma链已经完全敲除，和此文献中描述的表达IL-2受体gamma链截短体的品系有所区别: "Modulation of hematopoiesis in mice with a truncated mutant of the interleukin-2 receptor gamma chain" Ohbo K et al. Blood 1996. 87:956-67.
查看JAX的 NSG™ 系列小鼠模型资源 ，包括
• NSG™ 系列小鼠模型
• NSG™ 小鼠的饲养和育种注意事项
• NSG™ 常见问题
• 使用 NSG™ 的突破性研究
• 免疫缺陷品小鼠以及肿瘤移植物宿主的比较
• JAX免疫缺陷品系的免疫表型流式分析数据
参考文献
精选参考文献
如果在文章中使用了NSG小鼠品系，请引用原始文献并在材料与方法部分注明JAX品系号#005557。
如果在文章中使用了NSG小鼠品系，请引用原始文献并在材料与方法部分注明JAX品系号#005557。
2016
Myeloid Engraftment in Humanized Mice: Impact of Granulocyte-Colony Stimulating Factor Treatment and Transgenic Mouse Strain.
Coughlan AM , et al.
2005
Human lymphoid and myeloid cell development in NOD/LtSz-scid IL2R gamma null mice engrafted with mobilized human hemopoietic stem cells.
Shultz LD, et al.
健康报告 屏障设施级别描述 >
育种须知 更多繁育和饲养支持 >
维持活体种群时，可将 Prkdcscid 、 Il2rgtm1Wjl纯合的雌性小鼠与 Prkdcscid 纯合、Il2rgtm1Wjl 半合雄性小鼠杂交（Il2rg 为 X 连锁基因）。此过程建议在无特定病原体（specific pathogen-free, SPF）的条件下进行。
销售和使用条款
005557
NOD-scid IL2Rgammanull, NOD-scid IL2Rgnull, NSG, NOD scid gamma
肿瘤免疫，免疫学，感染类疾病，再生医学等研究领域
参考文献|
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精选参考文献如果在文章中使用了NSG小鼠品系，请引用原始文献并在材料与方法部分注明JAX品系号#005557。
2016Myeloid Engraftment in Humanized Mice: Impact of Granulocyte-Colony Stimulating Factor Treatment and Transgenic Mouse Strain.
Coughlan AM , et al.
2005Human lymphoid and myeloid cell development in NOD/LtSz-scid IL2R gamma null mice engrafted with mobilized human hemopoietic stem cells.
Shultz LD , et al. -
其他参考文献
2014NOD-scidIl2rg (tm1Wjl) and NOD-Rag1 (null) Il2rg (tm1Wjl) : a model for stromal cell-tumor cell interaction for human colon cancer.
Maykel J , et al.
2010Adult T-cell leukemia/lymphoma development in HTLV-1-infected humanized SCID mice.
Banerjee P , et al.
2010Aryl hydrocarbon receptor antagonists promote the expansion of human hematopoietic stem cells.
Boitano AE , et al.
2010Revised map of the human progenitor hierarchy shows the origin of macrophages and dendritic cells in early lymphoid development.
Doulatov S , et al.
2010Identification of a cell of origin for human prostate cancer.
Goldstein AS , et al.
2010CD8+ cell depletion accelerates HIV-1 immunopathology in humanized mice.
Gorantla S , et al.
2010Bortezomib induces apoptosis in primitive chronic myeloid leukemia cells including LTC-IC and NOD/SCID repopulating cells.
Heaney NB , et al.
2010Human hematopoietic stem/progenitor cells modified by zinc-finger nucleases targeted to CCR5 control HIV-1 in vivo.
Holt N , et al.
2010Effective and selective targeting of leukemia cells using a TORC1/2 kinase inhibitor.
Janes MR , et al.
2010Humanized nonobese diabetic-scid IL2rgammanull mice are susceptible to lethal Salmonella Typhi infection.
Libby SJ , et al.
2010Progenitor cell dose determines the pace and completeness of engraftment in a xenograft model for cord blood transplantation.
Liu C , et al.
2010Acute T-cell leukemias remain dependent on Notch signaling despite PTEN and INK4A/ARF loss.
Medyouf H , et al.
2010High frequencies of leukemia stem cells in poor-outcome childhood precursor-B acute lymphoblastic leukemias.
Morisot S , et al.
2010Compounds that target host cell proteins prevent varicella-zoster virus replication in culture, ex vivo, and in SCID-Hu mice.
Rowe J , et al.
2010Generation of functional human T-cell subsets with HLA-restricted immune responses in HLA class I expressing NOD/SCID/IL2r gamma(null) humanized mice.
Shultz LD , et al.
2010Human NK cells of mice with reconstituted human immune system components require preactivation to acquire functional competence.
Strowig T , et al.
2010AML xenograft efficiency is significantly improved in NOD/SCID-IL2RG mice constitutively expressing human SCF, GM-CSF and IL-3.
Wunderlich M , et al.
2009Expression of human cytokines dramatically improves reconstitution of specific human-blood lineage cells in humanized mice.
Chen Q , et al.
2009Busulfan produces efficient human cell engraftment in NOD/LtSz-Scid IL2Rgamma(null) mice.
Hayakawa J , et al.
2009CD47 is upregulated on circulating hematopoietic stem cells and leukemia cells to avoid phagocytosis.
Jaiswal S , et al.
2009Dengue virus infection and virus-specific HLA-A2 restricted immune responses in humanized NOD-scid IL2rgammanull mice.
Jaiswal S , et al.
2009Human peripheral blood leucocyte non-obese diabetic-severe combined immunodeficiency interleukin-2 receptor gamma chain gene mouse model of xenogeneic graft-versus-host-like disease and the role of host major histocompatibility complex.
King MA , et al.
2009Use of humanized severe combined immunodeficient mice for human vaccine development.
Koo GC , et al.
2009Comparison of human fetal liver, umbilical cord blood, and adult blood hematopoietic stem cell engraftment in NOD-scid/gammac-/-, Balb/c-Rag1-/-gammac-/-, and C.B-17-scid/bg immunodeficient mice.
Lepus CM , et al.
2009CD47 is an adverse prognostic factor and therapeutic antibody target on human acute myeloid leukemia stem cells.
Majeti R , et al.
2009High diversity of the immune repertoire in humanized NOD.SCID.gamma c-/- mice.
Marodon G , et al.
2009Humanized mice show clinical signs of dengue fever according to infecting virus genotype.
Mota J , et al.
2009A robust xenotransplantation model for acute myeloid leukemia.
Sanchez PV , et al.
2009Priming of protective T cell responses against virus-induced tumors in mice with human immune system components.
Strowig T , et al.
2009Sepsis-induced human lymphocyte apoptosis and cytokine production in 'humanized' mice.
Unsinger J , et al.
2009Suppression of xenogeneic graft-versus-host disease by treatment with immunoglobulin-like transcript 3-Fc.
Vlad G , et al.
2008Human acute leukemia cells injected in NOD/LtSz-scid/IL-2Rgamma null mice generate a faster and more efficient disease compared to other NOD/scid-related strains.
Agliano A , et al.
2008Acute myeloid leukemia (AML)-reactive cytotoxic T lymphocyte clones rapidly expanded from CD8(+) CD62L((high)+) T cells of healthy donors prevent AML engraftment in NOD/SCID IL2Rgamma(null) mice.
Distler E , et al.
2008Expanded CD34+ human umbilical cord blood cells generate multiple lymphohematopoietic lineages in NOD-scid IL2rgamma(null) mice.
Giassi LJ , et al.
2008The differentiative and regenerative properties of human hematopoietic stem/progenitor cells in NOD-SCID/IL2rgamma(null) mice.
Ishikawa F , et al.
2008Humanized mice for the study of type 1 diabetes and beta cell function.
King M , et al.
2008A new Hu-PBL model for the study of human islet alloreactivity based on NOD-scid mice bearing a targeted mutation in the IL-2 receptor gamma chain gene.
King M , et al.
2008CD34+CD38+CD19+ as well as CD34+CD38-CD19+ cells are leukemia-initiating cells with self-renewal capacity in human B-precursor ALL.
Kong Y , et al.
2008T cell-specific siRNA delivery suppresses HIV-1 infection in humanized mice.
Kumar P , et al.
2008In childhood acute lymphoblastic leukemia, blasts at different stages of immunophenotypic maturation have stem cell properties.
le Viseur C , et al.
2008Creation of 'humanized' mice to study human immunity.
Pearson T , et al.
2008A new immunodeficient hyperglycaemic mouse model based on the Ins2 ( Akita ) mutation for analyses of human islet and beta stem and progenitor cell function.
Pearson T , et al.
2008Non-obese diabetic-recombination activating gene-1 (NOD-Rag1 null) interleukin (IL)-2 receptor common gamma chain (IL2r gamma null) null mice: a radioresistant model for human lymphohaematopoietic engraftment.
Pearson T , et al.
2008Efficient tumour formation by single human melanoma cells.
Quintana E , et al.
2008Long-term engraftment and expansion of tumor-derived memory T cells following the implantation of non-disrupted pieces of human lung tumor into NOD-scid IL2Rgamma(null) mice.
Simpson-Abelson MR , et al.
2007The developmental program of human dendritic cells is operated independently of conventional myeloid and lymphoid pathways.
Ishikawa F , et al.
2007Chemotherapy-resistant human AML stem cells home to and engraft within the bone-marrow endosteal region.
Ishikawa F , et al.
2007Identification of a hierarchy of multipotent hematopoietic progenitors in human cord blood.
Majeti R , et al.
2007Humanized mice in translational biomedical research.
Shultz LD , et al.
2007Humanized NOD/LtSz-scid IL2 receptor common gamma chain knockout mice in diabetes research.
Shultz LD , et al.
2006Purified human hematopoietic stem cells contribute to the generation of cardiomyocytes through cell fusion.
Ishikawa F , et al.
2005Development of functional human blood and immune systems in NOD/SCID/IL2 receptor {gamma} chain(null) mice.
Ishikawa F , et al.
