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NOD.Cg-Prkdcscid/J
品系货号:001303 | 通用名称:NOD scid
又名: NOD SCID
摘要
此品系为严重联合免疫缺陷自发突变 Prkdcscid 纯合小鼠,通常称为 scid 小鼠,表现为功能性 T 细胞和 B 细胞缺失、淋巴细胞减少症、低丙种球蛋白血症,但造血微环境正常。Prkdcscid 小鼠可以接受同种异体和异种异体移植物,因此是细胞转移实验的理想模型。总体而言,NOD/ShiLtSz 遗传背景下的 Prkdcscid渗漏表达较低。
品系特点
自发突变
  
原始参考文献
Info 如果研究中使用了NOD scid小鼠品系,请在发表文章时请引用原始文献并在材料和方法部分提及JAX品系号001303。
查阅参考文献 >
  
品系详情
此品系为严重联合免疫缺陷自发突变 Prkdcscid 纯合小鼠,通常称为 scid 小鼠,表现为功能性 T 细胞和 B 细胞缺失、淋巴细胞减少症、低丙种球蛋白血症,但造血微环境正常。抗原呈递细胞、髓系细胞和 NK 细胞的正常功能具有品系依赖性。scid 小鼠携带 DNA 修复缺陷和编码淋巴细胞中抗原特异性受体的基因重排缺陷。大多数纯合子未检出 IgM、IgG1、IgG2a、IgG2b、IgG3 或 IgA。胸腺、淋巴结和脾脏滤泡中几乎没有淋巴细胞。scid 小鼠可以接受同种异体和异种异体移植物,因此是细胞转移实验的理想模型。
有些 scid 小鼠会自发出现局部免疫反应。血清 Ig 水平高于 1 ug/ml 的 scid 小鼠被视为“渗漏表达”。scid渗漏具有高度品系依赖性、随周龄增加,在非 SPF 条件下笼养的小鼠中比例更高。总体而言,scid 渗漏率在 C57BL/6J 和 BALB/cBy 遗传背景中较高,在 C3H/HeJ 背景中较低,在 NOD/ShiLtSz 背景中更低。NOD/ShiLtSz-Prkdcscid 小鼠终生不患胰岛炎和糖尿病,可作为NOD/ShiLtJ 小鼠(品系货号 001976)的无糖尿病对照。不过,其胸腺淋巴瘤发病率较高,在无特定病原条件下(SPF),寿命通常仅限于 8.5 月龄。NOD.CB17-Prkdcscid/J 小鼠除可用作异体移植的理想宿主外,还有可用于研究T 细胞亚群在自身免疫性糖尿病中的作用,同时也可作为无胰岛炎胰岛细胞的来源。
品系建立
Prkdcscid 突变是在宾夕法尼亚州费城癌症研究所饲养的 BALB/c-Ighb (CB-17) 小鼠种群中自发产生的突变。将 Prkdcscid 突变按照如下描述回交至 NOD/ShiLtSz 背景中:使 NOD/ShiLtSz 雌鼠与 C.B-17-Prkdcscid 雄鼠交配;使 F1/N1 和随后数代 Prkdcscid/+ 雄鼠后代与 NOD/ShiLtSz 雌鼠交配,总共与 NOD/ShiLtSz 回交 10 代;在 N10 代时,通过兄弟姊妹近亲交配得到 Prkdcscid 纯合小鼠。
参考文献
精选参考文献
如果研究中使用了NOD scid小鼠品系,请在发表文章时请引用原始文献并在材料和方法部分提及JAX品系号001303。
1998

Xenotransplantation of human lymphoid malignancies is optimized in mice with multiple immunologic defects.

Hudson WA , et al.

PubMed:9844934

MGI:J:153650

Leukemia 12(12):2029-33

1996

Identification of a nonsense mutation in the carboxyl-terminal region of DNA-dependent protein kinase catalytic subunit in the scid mouse.

Blunt T , et al.

PubMed:8816792

MGI:J:35393

Proc Natl Acad Sci U S A 93(19):10285-90

1995

Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation.

Blunt T , et al.

PubMed:7889575

MGI:J:30365

Cell 80(5):813-23

基因分型和繁育
See control note:

近似对照

Allele Symbol Prkdc<scid>
Allele Specific Protocols
 
此品系繁育表现良好。
繁育策略
纯合子X纯合子
销售和使用条款

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001303
NOD scid
肿瘤免疫,癌症研究,免疫相关研究,糖尿病及肥胖
参考文献|
x
  •  
    精选参考文献
    如果研究中使用了NOD scid小鼠品系,请在发表文章时请引用原始文献并在材料和方法部分提及JAX品系号001303。

    1998
    Xenotransplantation of human lymphoid malignancies is optimized in mice with multiple immunologic defects.
    Hudson WA , et al.
    PubMed:9844934
    MGI:J:153650
    Leukemia 12(12):2029-33

    1996
    Identification of a nonsense mutation in the carboxyl-terminal region of DNA-dependent protein kinase catalytic subunit in the scid mouse.
    Blunt T , et al.
    PubMed:8816792
    MGI:J:35393
    Proc Natl Acad Sci U S A 93(19):10285-90

    1995
    Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation.
    Blunt T , et al.
    PubMed:7889575
    MGI:J:30365
    Cell 80(5):813-23

    1995
    Emv30null NOD-scid mice. An improved host for adoptive transfer of autoimmune diabetes and growth of human lymphohematopoietic cells.
    Serreze DV , et al.
    PubMed:7589844
    MGI:J:29951
    Diabetes 44(12):1392-8

    1995
    Multiple defects in innate and adaptive immunologic function in NOD/LtSz-scid mice.
    Shultz LD , et al.
    PubMed:7995938
    MGI:J:22026
    J Immunol 154(1):180-91

    1993
    Adoptive transfer of diabetes into immunodeficient NOD-scid/scid mice. Relative contributions of CD4+ and CD8+ T-cells from diabetic versus prediabetic NOD.NON-Thy-1a donors.
    Christianson SW , et al.
    PubMed:8093606
    MGI:J:34990
    Diabetes 42(1):44-55

    1993
    High frequency of thymic ectopy in thyroids from autoimmune prone nonobese diabetic female mice.
    Many MC , et al.
    PubMed:8377477
    MGI:J:14747
    Lab Invest 69(3):364-7

    1993
    Strain-dependent leakiness of mice with severe combined immune deficiency.
    Nonoyama S , et al.
    PubMed:8473734
    MGI:J:4610
    J Immunol 150(9):3817-24

    1992
    The nonobese diabetic scid mouse: model for spontaneous thymomagenesis associated with immunodeficiency.
    Prochazka M , et al.
    PubMed:1373493
    MGI:J:513
    Proc Natl Acad Sci U S A 89(8):3290-4

    1988
    The scid mouse mutant.
    Bosma M , et al.
    PubMed:3416632
    MGI:J:9341
    Curr Top Microbiol Immunol 137:197-202

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    其它参考文献

    2014
    NOD-scidIl2rg (tm1Wjl) and NOD-Rag1 (null) Il2rg (tm1Wjl) : a model for stromal cell-tumor cell interaction for human colon cancer.
    Maykel J , et al.
    PubMed:24798995
    MGI:J:227975
    Dig Dis Sci 59(6):1169-79

    2004
    T-cell receptor transgenic response to an endogenous polymorphic autoantigen determines susceptibility to diabetes.
    Pauza ME , et al.
    PubMed:15047613
    MGI:J:89082
    Diabetes 53(4):978-88

    2003
    Tim-3 inhibits T helper type 1-mediated auto- and alloimmune responses and promotes immunological tolerance.
    Sanchez-Fueyo A , et al.
    PubMed:14556005
    MGI:J:86260
    Nat Immunol 4(11):1093-101

    2003
    Contribution of Fas to diabetes development.
    Savinov AY , et al.
    PubMed:12525697
    MGI:J:81416
    Proc Natl Acad Sci U S A 100(2):628-32

    2003
    Non-obese diabetic mice rapidly develop dramatic sympathetic neuritic dystrophy: a new experimental model of diabetic autonomic neuropathy.
    Schmidt RE , et al.
    PubMed:14578206
    MGI:J:86318
    Am J Pathol 163(5):2077-91

    2002
    Indoleamine 2,3-dioxygenase expression in transplanted NOD Islets prolongs graft survival after adoptive transfer of diabetogenic splenocytes.
    Alexander AM , et al.
    PubMed:11812742
    MGI:J:74301
    Diabetes 51(2):356-65

    2002
    Susceptibility to lymphoid neoplasia in immunodeficient strains of nonobese diabetic mice.
    Chiu PP , et al.
    PubMed:12384545
    MGI:J:79669
    Cancer Res 62(20):5828-34

    2002
    Congenic mapping of the diabetogenic locus Idd4 to a 5.2-cM region of chromosome 11 in NOD mice: identification of two potential candidate subloci.
    Grattan M , et al.
    PubMed:11756344
    MGI:J:73763
    Diabetes 51(1):215-23

    2002
    Elimination of maternally transmitted autoantibodies prevents diabetes in nonobese diabetic mice.
    Greeley SA , et al.
    PubMed:11927947
    MGI:J:75803
    Nat Med 8(4):399-402

    2002
    Regulation of diabetes development by regulatory T cells in pancreatic islet antigen-specific TCR transgenic nonobese diabetic mice.
    Kanagawa O , et al.
    PubMed:12055228
    MGI:J:89793
    J Immunol 168(12):6159-64

    2001
    Previously undetected human hematopoietic cell populations with short-term repopulating activity selectively engraft NOD/SCID-beta2 microglobulin-null mice.
    Glimm H , et al.
    PubMed:11160136
    MGI:J:66974
    J Clin Invest 107(2):199-206

    2001
    Human homologues of Delta-1 and Delta-4 function as mitogenic regulators of primitive human hematopoietic cells.
    Karanu FN , et al.
    PubMed:11264159
    MGI:J:68395
    Blood 97(7):1960-7

    2001
    Altered myelopoiesis and the development of acute myeloid leukemia in transgenic mice overexpressing cyclin A1.
    Liao C , et al.
    PubMed:11381140
    MGI:J:69909
    Proc Natl Acad Sci U S A 98(12):6853-8

    2001
    Genetic interactions between ATM and the nonhomologous end-joining factors in genomic stability and development.
    Sekiguchi J , et al.
    PubMed:11248063
    MGI:J:68074
    Proc Natl Acad Sci U S A 98(6):3243-8

    2001
    Il-12 administration reveals diabetogenic t cells in genetically resistant i-ealpha-transgenic nonobese diabetic mice: resistance to autoimmune diabetes is associated with binding of ealpha-derived peptides to the i-a(g7) molecule.
    Trembleau S , et al.
    PubMed:11564833
    MGI:J:71651
    J Immunol 167(7):4104-14

    2000
    Porcine stem cell engraftment and seeding of murine thymus with class II+ cells in mice expressing porcine cytokines: toward tolerance induction across discordant xenogeneic barriers.
    Chen AM , et al.
    PubMed:10910267
    MGI:J:93486
    Transplantation 69(12):2484-90

    2000
    Thymic and postthymic regulation of diabetogenic CD8 T cell development in TCR transgenic nonobese diabetic (NOD) mice.
    Kanagawa O , et al.
    PubMed:10799914
    MGI:J:62643
    J Immunol 164(10):5466-73

    1993
    Granulosa cell tumorigenesis in genetically hypogonadal-immunodeficient mice grafted with ovaries from tumor-susceptible donors.
    Beamer WG , et al.
    PubMed:8339285
    MGI:J:14443
    Cancer Res 53(16):3741-6

    1985
    Severe combined immunodeficiency (SCID) in the mouse. Pathology, reconstitution, neoplasms.
    Custer RP , et al.
    PubMed:2412448
    MGI:J:30980
    Am J Pathol 120(3):464-77

    1984
    Functional status of cells from lymphoid and myeloid tissues in mice with severe combined immunodeficiency disease.
    Dorshkind K , et al.
    PubMed:6607948
    MGI:J:7343
    J Immunol 132(4):1804-8

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    等位基因参考文献



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Related Genotype: A/A Tyrc/Tyrc