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B6.129P2-Apoetm1Unc/J
品系货号：002052 ｜ 通用名称：ApoE KO 小鼠
摘要
此品系 Apoetm1Unc 基因敲除突变纯合小鼠的血浆总胆固醇水平明显升高，且不受鼠龄或性别影响。 3 月龄小鼠的近端主动脉可见脂纹。该病变随鼠龄增大而加剧，逐渐发展为脂质含量更低但细胞更长的病变，这是动脉粥样硬化病变前的后期典型特征。这些 ApoE KO 小鼠适用于研究心血管疾病、动脉粥样硬化和脂肪代谢，以及研究 ApoE 在阿尔茨海默症中的作用。
品系特点
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同源品系
靶向突变
品系捐赠者 Nobuyo Maeda博士 北卡罗莱纳大学教堂山分校
原始参考文献
当使用该小鼠品系发表文献时，请引用原始参考文献，并在材料方法中提供该品系的品系货号：JAX stock #002052
查阅参考文献 > 当使用该小鼠品系发表文献时，请引用原始参考文献，并在材料方法中提供该品系的品系货号：JAX stock #002052品系详情
此品系Apoetm1Unc 突变纯合小鼠的血浆总胆固醇水平明显升高，且不受鼠龄或性别的影响。 3 月龄小鼠的近端主动脉可见脂纹。该病变随鼠龄增大而加剧，逐渐发展为脂质含量更低但细胞更长的病变，这是动脉粥样硬化病变前的后期典型特征。在 C57BL/6 x 129 混合遗传背景上具有该突变的小鼠甘油三酯水平中度升高。研究显示，apoE 缺陷老龄鼠（> 17 月龄）的大脑中会出现黄色瘤样病变，该病变主要包括结晶胆固醇裂隙、脂质小球和泡沫细胞。脉络丛和腹穹窿中可见较小黄色瘤。另有研究表明，apoE 缺陷小鼠的应激反应发生改变、空间学习和记忆受损、长时程增强效应改变，且突触受损。
品系建立
Apoetm1Unc 突变品系由北卡罗来纳大学教堂山分校的 Nobuyo Maeda 博士实验室建立。在品系建立过程中，使用了源自 129P2/OlaHsd 的 E14Tg2a ES 细胞系。所用质粒命名为 pNMC109，始祖细胞系为主要参考文献中提及的 T-89。此 C57BL/6J 同源 Apoetm1Unc 品系是通过与 C57BL/6J 近交小鼠回交至少 10 代获得的。这些小鼠的被毛呈黑色。
需要注意的是，在 1995 年以前，仅销售与 C57BL/6J 小鼠回交 6 代 (N6) 的小鼠。N6 代小鼠为粉红色眼睛稀释基因 p 的纯合小鼠，该基因使此品系小鼠具有粉红色眼睛和银色被毛。E14Tg2a ES 细胞系携带此种隐性突变，在回交 N6 代时，该突变仍与 7 号染色体上的靶向 Apoe 基因连锁。目前，已不再销售 N6 种群小鼠 [1995]。
参考文献
精选参考文献
当使用该小鼠品系发表文献时，请引用原始参考文献，并在材料方法中提供该品系的品系货号：JAX stock #002052
1992
Generation of mice carrying a mutant apolipoprotein E gene inactivated by gene targeting in embryonic stem cells.
Piedrahita JA , et al.
健康报告 屏障设施级别描述 >
育种须知 更多繁育和饲养支持 >
此品系繁育表现良好。
繁育策略
纯合子x纯合子
销售和使用条款
法务咨询
电话： 001-207-288-6470（美国）
电子邮件： TechTran@jax.org
002052
ApoE KO 小鼠
神经生物学，糖尿病与肥胖症，心血管疾病
参考文献|
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精选参考文献当使用该小鼠品系发表文献时，请引用原始参考文献，并在材料方法中提供该品系的品系货号：JAX stock #002052
1992Generation of mice carrying a mutant apolipoprotein E gene inactivated by gene targeting in embryonic stem cells.
Piedrahita JA , et al. -
其它参考文献
2004Neuron-specific apolipoprotein e4 proteolysis is associated with increased tau phosphorylation in brains of transgenic mice.
Brecht WJ , et al.
2004A nontransgenic mouse model shows inducible amyloid-beta (Abeta) peptide deposition and elucidates the role of apolipoprotein E in the amyloid cascade.
Dolev I , et al.
2004Effect of macrophage-derived apolipoprotein E on hyperlipidemia and atherosclerosis of LDLR-deficient mice.
Shi W , et al.
2003A decreased expression of angiopoietin-like 3 is protective against atherosclerosis in apoE-deficient mice.
Ando Y , et al.
2003Loss of TGF-beta1 leads to increased neuronal cell death and microgliosis in mouse brain.
Brionne TC , et al.
2003Angiotensin II-accelerated atherosclerosis and aneurysm formation is attenuated in osteopontin-deficient mice.
Bruemmer D , et al.
2003Two Hsp70 family members expressed in atherosclerotic lesions.
Han Z , et al.
2003Circulating activated platelets exacerbate atherosclerosis in mice deficient in apolipoprotein E.
Huo Y , et al.
2003Impaired electroretinogram (ERG) response in apolipoprotein E-deficient mice.
Ong JM , et al.
2003Central role of RAGE-dependent neointimal expansion in arterial restenosis.
Sakaguchi T , et al.
2003Hyperhomocysteinemia accelerates atherosclerosis in cystathionine beta-synthase and apolipoprotein E double knock-out mice with and without dietary perturbation.
Wang H , et al.
2003Supplementation of diets with the black rice pigment fraction attenuates atherosclerotic plaque formation in apolipoprotein e deficient mice.
Xia M , et al.
2002Hypobetalipoproteinemic Mice with a Targeted Apolipoprotein (Apo) B-27.6-specifying Mutation. IN VIVO EVIDENCE FOR AN IMPORTANT ROLE OF AMINO ACIDS 1254-1744 OF ApoB IN LIPID TRANSPORT AND METABOLISM OF THE ApoB-CONTAINING LIPOPROTEIN.
Chen Z , et al.
2002Dietary restriction reduces atherosclerosis and oxidative stress in the aorta of apolipoprotein E-deficient mice.
Guo Z , et al.
2002Failure of red blood cell maturation in mice with defects in the high-density lipoprotein receptor SR-BI.
Holm TM , et al.
2002The ATP binding cassette transporter A1 (ABCA1) modulates the development of aortic atherosclerosis in C57BL/6 and apoE-knockout mice.
Joyce CW , et al.
2002LDL receptor but not apolipoprotein E deficiency increases diet-induced obesity and diabetes in mice.
Schreyer SA , et al.
2002Loss of Lymphotoxin-alpha but Not Tumor Necrosis Factor-alpha Reduces Atherosclerosis in Mice.
Schreyer SA , et al.
2002Reduction of blood pressure, plasma cholesterol, and atherosclerosis by elevated endothelial nitric oxide.
van Haperen R , et al.
2001Atherosclerosis in apoE knockout mice infected with multiple pathogens.
Burnett MS , et al.
2001The adhesion receptor CD44 promotes atherosclerosis by mediating inflammatory cell recruitment and vascular cell activation.
Cuff CA , et al.
2001Dietary gamma-linolenic acid suppresses aortic smooth muscle cell proliferation and modifies atherosclerotic lesions in apolipoprotein E knockout mice.
Fan YY , et al.
2001Apoprotein C-III deficiency markedly stimulates triglyceride secretion in vivo: comparison with apoprotein E.
Hirano T , et al.
2001Retrovirus-mediated expression of apolipoprotein A-I in the macrophage protects against atherosclerosis in vivo.
Ishiguro H , et al.
2001Mixed allogeneic chimerism with wild-type strains ameliorates atherosclerosis in apolipoprotein E-deficient mice.
Ishimori N , et al.
2001Iron overload diminishes atherosclerosis in apoE-deficient mice.
Kirk EA , et al.
2001Compared with saturated fatty acids, dietary monounsaturated fatty acids and carbohydrates increase atherosclerosis and VLDL cholesterol levels in LDL receptor-deficient, but not apolipoprotein E-deficient, mice.
Merkel M , et al.
2001Tea catechins prevent the development of atherosclerosis in apoprotein E-deficient mice.
Miura Y , et al.
2001Pathophysiology of apolipoprotein E deficiency in mice: relevance to apo E-related disorders in humans.
Moghadasian MH , et al.
2001ApoE(-/-) mice develop atherosclerosis in the absence of complement component C5.
Patel S , et al.
2001Impact of apoE deficiency on oxidative insults and antioxidant levels in the brain.
Ramassamy C , et al.
2001Effect of azithromycin on murine arteriosclerosis exacerbated by Chlamydia pneumoniae.
Rothstein NM , et al.
2000Targeted disruption of the class B scavenger receptor CD36 protects against atherosclerotic lesion development in mice [see comments]
Febbraio M , et al.
2000Age-dependent cytokine responses: trimethyltin hippocampal injury in wild-type, APOE knockout, and APOE4 mice.
Harry GJ , et al.
2000Role of endogenous nitric oxide in progression of atherosclerosis in apolipoprotein E-deficient mice.
Kauser K , et al.
2000Combined serum paraoxonase knockout/apolipoprotein E knockout mice exhibit increased lipoprotein oxidation and atherosclerosis.
Shih DM , et al.
1998The brain renin-angiotensin system contributes to the hypertension in mice containing both the human renin and human angiotensinogen transgenes.
Davisson RL , et al.
1998Apolipoprotein-E deficiency results in an altered stress responsiveness in addition to an impaired spatial memory in young mice.
Zhou Y , et al.
1994The two-receptor model of lipoprotein clearance: tests of the hypothesis in knockout mice lacking the low density lipoprotein receptor, apolipoprotein E, or both proteins.
Ishibashi S , et al.
1992Spontaneous hypercholesterolemia and arterial lesions in mice lacking apolipoprotein E.
Zhang SH , et al.
