C57BL/6J
品系货号:000664 | 通用名称:B6
又名:B6J, B6/J
摘要
C57BL/6J是应用最广泛的近交系,也是第一个完成全基因组测序的近交系小鼠。虽然该品系对多种肿瘤耐受,但它被广泛用作表达突变基因的遗传背景品系。C57BL/6J小鼠对听源性癫痫发作有抗性,骨密度相对较低,并出现与年龄相关的听力丧失。它们还易患由饮食诱导的肥胖、2型糖尿病和动脉粥样硬化。该品系的巨噬细胞对炭疽致死毒素的作用具有抗性。我们同时提供可即刻用于研究的25-90周龄的C57BL/6J大龄或老龄雌鼠和雄鼠
重要提示:该品系是Cdh23ahl的纯合子,Cdh23ahl是一种与年龄相关的听力损失1突变,导致10月龄后发生进行性听力丧失。
品系特点
原始参考文献
Info 当使用该小鼠品系发表文献时,请引用原始文献,并在材料方法中提供该品系的品系货号:JAX stock#000664
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品系详情

 C57BL/6J是使用最广泛的近交小鼠品系。它通常作为常规品系使用,也被用于构建携带自发和诱导突变的同源系小鼠的背景品系。虽然此品系对多种肿瘤耐受,但它被普遍接受作为表达大部分突变基因的遗传背景。C57BL/6J 小鼠被用于多种研究领域,包括心血管生物学、发育生物学、糖尿病和肥胖、遗传学、免疫学、神经生物学和感觉神经研究。C57BL/6J 小鼠也常被用于转基因小鼠的构建。总体上,C57BL/6J 小鼠繁殖良好,寿命长,对肿瘤易感性低。与 BALB/C 和 DBA/2J 小鼠相比,C57BL/6J 小鼠的原始造血干细胞表现出明显的衰老延迟。这是一个显性性状。其他特征包括:1) 对膳食诱导的肥胖、II 型糖尿病和动脉硬化症高度易感;2) 小眼畸形和其他相关眼部异常发病率高;3) 对听源性惊厥有抗性;4) 骨密度低;5) 遗传性脑积水(早期报告显示出现概率为 1-4%);6) 门体分流(约 5%);7) 与过度理毛相关的脱毛;8) 酒精和吗啡偏好;9) 迟发性听力丧失;10) 脑积水和咬合不正发病率增加。

 

喂食高脂饲料的 C57BL/6J小鼠出现肥胖、轻度至中度高血糖和高胰岛素血症(见 JAX® 饮食诱导的肥胖 (DIO) 模型)。C57BL/6J 小鼠喂食致动脉粥样硬化饲料(1.25% 胆固醇、0.5% 胆酸和 15% 脂肪)14 周,出现 4500 至 8000 μm2 动脉粥样硬化主动脉病变/主动脉横截面病变。不同近交品系中主动脉病变的多样性导致了 8 个影响动脉硬化症的基因 Ath1Ath8 的发现。由于 Cdh23Ahl 等位基因的存在,C57BL/6J 小鼠会随着外毛细胞和内毛细胞的破坏发展出严重且进行性的听力丧失。Cheers 和 McKenzie 发现 C57BL/6J 小鼠对李氏杆菌病耐受。1984 年之前的某个时间,C57BL/6J 小鼠的烟酰胺核苷酸转移酶 (Nnt) 外显子 7-11 发生了自然缺失。这种缺失导致 NNT 蛋白的缺失,与葡萄糖内稳态控制受损和胰岛素分泌减少相关。此突变不存在于 C57BL/6JEi、C57BL/6N、C57BL/6NJ、C57BL/6ByJ、C57BL/10J、C57L/J 或 C58/J 小鼠中 (Toye AA, et al, Diabetologia, 2005)。由于品系 C57BL/6JEi 是在 1976 年从 C57BL/6J 中分离出来的,Nnt 缺失应发生在 1976 年至 1984 年之间。由于存在 n-Tr20m1J 点突变(该点突变也存在于 C57BL/6JEiJ 小鼠中,但在 C57BL/6NJ 或 C57BL/6ByJ 小鼠中不存在),与其他近交品系相比,C57BL/6J 小鼠的小脑提取物在 AGA 密码子处的核糖体停顿增加 (Ishimura et al., 2014)。此外,20 世纪 70 年代早期至 20 世纪 90 年代之间的某个时间发生在 Gabra2 中的内含子点缺失导致大脑中该氯离子通道组分的转录和蛋白表达降低。

 

C57BL/6J 是国际合作进行第一个高质量小鼠基因组草图序列测序的 DNA 来源。已有 5 个可区分 C57BL/6J 与 C57BL/6ByJ 和 C57BL/6NJ 品系的 SNP 被鉴定。C57BL/6ByJ 和 C57BL/6NJ 分型如下:08-015199792-M (rs3709624) 是 C;11-004367508-M (rs3659787) 是 A;13-041017317-M (rs3722313) 是 C;15-057561875-M (rs3702158) 是 G;19-049914266-M (rs3724876) 是 T。C57BL/6J 分型如下:08-015199792-M (rs3709624) 是 T;11-004367508-M (rs3659787) 是 G;13-041017317-M (rs3722313) 是 T;15-057561875-M (rs3702158) 是 A;19-049914266-M (rs3724876) 是 G(Petkov 和 Wiles, 2004.)其他人随后鉴定了 C57BL/6 的亚系之间的更多 SNP 差异(Mekada et al., 2009、Zurita et al., 2010)。

品系建立
C57BL/6J 近交品系由 CC Little 博士将来自 Abbie Lathrop 女士的储备库的 57 号雌鼠与 52 号雄鼠交配繁育获得。这一交配繁育也产生了 C57L 和 C57BR 品系。
参考文献
精选参考文献  
当使用该小鼠品系发表文献时,请引用原始文献,并在材料方法中提供该品系的品系货号:JAX stock#000664
2020

Expression of the Neuronal tRNA n-Tr20 Regulates Synaptic Transmission and Seizure Susceptibility.

Kapur M , et al.

PubMed:32853550

MGI:J:297745

Neuron 108(1):193-208.e9

2016

Attention to Background Strain Is Essential for Metabolic Research: C57BL/6 and the International Knockout Mouse Consortium.

Fontaine DA , et al.

PubMed:26696638

MGI:J:231066

Diabetes 65(1):25-33

2013

A comparative phenotypic and genomic analysis of C57BL/6J and C57BL/6N mouse strains.

Simon MM , et al.

PubMed:23902802

MGI:J:220417

Genome Biol 14(7):R82

基因分型和繁育
Allele Symbol A<w>
Allele Specific Protocols
Allele Symbol Gabra2<C57BL/6J>
Allele Specific Protocols
Allele Symbol H2<b>
Allele Specific Protocols
Allele Symbol Nnt<C57BL/6J>
Allele Specific Protocols
该品系繁育表现出色
繁育策略
Sibling x Sibling (同胞兄弟姐妹交配)
销售和使用条款
 

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000664
B6, B6J, B6/J
常规品系,工具品系,发育,神经,心血管,代谢,老化等
参考文献|
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    精选参考文献
    当使用该小鼠品系发表文献时,请引用原始文献,并在材料方法中提供该品系的品系货号:JAX stock#000664

    2020
    Expression of the Neuronal tRNA n-Tr20 Regulates Synaptic Transmission and Seizure Susceptibility.
    Kapur M , et al.
    PubMed:32853550
    MGI:J:297745
    Neuron 108(1):193-208.e9

    2016
    Attention to Background Strain Is Essential for Metabolic Research: C57BL/6 and the International Knockout Mouse Consortium.
    Fontaine DA , et al.
    PubMed:26696638
    MGI:J:231066
    Diabetes 65(1):25-33

    2013
    A comparative phenotypic and genomic analysis of C57BL/6J and C57BL/6N mouse strains.
    Simon MM , et al.
    PubMed:23902802
    MGI:J:220417
    Genome Biol 14(7):R82

    2008
    Genetic background determines metabolic phenotypes in the mouse.
    Champy MF , et al.
    PubMed:18392653
    MGI:J:137669
    Mamm Genome 19(5):318-31

    2005
    A genetic and physiological study of impaired glucose homeostasis control in C57BL/6J mice.
    Toye AA , et al.
    PubMed:15729571
    MGI:J:106692
    Diabetologia 48(4):675-86

    2004
    Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse.
    Petkov PM , et al.
    PubMed:15081119
    MGI:J:89298
    Genomics 83(5):902-11

    2002
    Initial sequencing and comparative analysis of the mouse genome.
    Waterston RH , et al.
    PubMed:12466850
    MGI:J:80507
    Nature 420(6915):520-62

    2001
    Genetic loci determining bone density in mice with diet-induced atherosclerosis.
    Drake TA , et al.
    PubMed:11328966
    MGI:J:69682
    Physiol Genomics 5(4):205-15

    1998
    Differential effects of fat and sucrose on body composition in A/J and C57BL/6 mice.
    Black BL , et al.
    PubMed:9826212
    MGI:J:50965
    Metabolism 47(11):1354-9

    1991
    Effects of atherogenic diet consumption on lipoproteins in mouse strains C57BL/6 and C3H.
    Ishida BY , et al.
    PubMed:1856605
    MGI:J:109926
    J Lipid Res 32(4):559-68

    1985
    Variation in susceptibility to atherosclerosis among inbred strains of mice.
    Paigen B , et al.
    PubMed:3841001
    MGI:J:109950
    Atherosclerosis 57(1):65-73

  •  
    其他参考文献

    2016
    Unstable FoxP3+ T regulatory cells in NZW mice.
    Depis F , et al.
    PubMed:26768846
    MGI:J:227903
    Proc Natl Acad Sci U S A 113(5):1345-50

    2016
    Pathophysiological mechanisms underlying phenotypic differences in pulmonary radioresponse.
    Jackson IL , et al.

    2016
    Clinical Chemistry Reference Intervals for C57BL/6J, C57BL/6N, and C3HeB/FeJ Mice (Mus musculus).
    Otto GP , et al.
    PubMed:27423143
    MGI:J:262147
    J Am Assoc Lab Anim Sci 55(4):375-86

    2016
    Nlrp12 mutation causes C57BL/6J strain-specific defect in neutrophil recruitment.
    Ulland TK , et al.
    PubMed:27779193
    MGI:J:238047
    Nat Commun 7:13180

    2014
    RNA function. Ribosome stalling induced by mutation of a CNS-specific tRNA causes neurodegeneration.
    Ishimura R , et al.
    PubMed:25061210
    MGI:J:211326
    Science 345(6195):455-9

    2013
    C57BL/6N mutation in Cytoplasmic FMRP interacting protein 2 regulates cocaine response.
    Kumar V , et al.
    PubMed:24357318
    MGI:J:203183
    Science 342(6165):1508-12

    2011
    Genetic polymorphisms among C57BL/6 mouse inbred strains.
    Zurita E , et al.
    PubMed:20506040
    MGI:J:160203
    Transgenic Res 20(3):481-9

    2009
    Genetic differences among C57BL/6 substrains.
    Mekada K , et al.
    PubMed:19448337
    MGI:J:160204
    Exp Anim 58(2):141-9

    2009
    Comparison of allergic lung disease in three mouse strains after systemic or mucosal sensitization with ovalbumin antigen.
    Zhu W , et al.
    PubMed:19224206
    MGI:J:146790
    Immunogenetics 61(3):199-207

    2008
    Oxidative damage-induced inflammation initiates age-related macular degeneration.
    Hollyfield JG , et al.
    PubMed:18223656
    MGI:J:129432
    Nat Med 14(2):194-8

    2007
    Mouse behavioral tasks relevant to autism: phenotypes of 10 inbred strains.
    Moy SS , et al.
    PubMed:16971002
    MGI:J:138682
    Behav Brain Res 176(1):4-20

    2006
    Deletion of Nicotinamide Nucleotide Transhydrogenase: A New Quantitive Trait Locus Accounting for Glucose Intolerance in C57BL/6J Mice.
    Freeman HC , et al.
    PubMed:16804088
    MGI:J:109356
    Diabetes 55(7):2153-6

    2004
    An efficient SNP system for mouse genome scanning and elucidating strain relationships.
    Petkov PM , et al.
    PubMed:15342563
    MGI:J:151913
    Genome Res 14(9):1806-11

    2003
    Mouse strain differences in kainic acid sensitivity, seizure behavior, mortality, and hippocampal pathology.
    McKhann GM 2nd , et al.
    PubMed:14614919
    MGI:J:262175
    Neuroscience 122(2):551-61

    2003
    Variation in type 2 diabetes--related traits in mouse strains susceptible to diet-induced obesity.
    Rossmeisl M , et al.
    PubMed:12882911
    MGI:J:86027
    Diabetes 52(8):1958-66

    2003
    Allergen-induced airway disease is mouse strain dependent.
    Whitehead GS , et al.
    PubMed:12626335
    MGI:J:84265
    Am J Physiol Lung Cell Mol Physiol 285(1):L32-42

    2000
    Age-related morphometric changes in the pineal gland. A comparative study between C57BL/6J and CBA mice.
    Cernuda-Cernuda R , et al.
    PubMed:11005233
    MGI:J:240797
    Histol Histopathol 15(4):1087-92

    2000
    Genetic regulation of primitive hematopoietic stem cell senescence.
    Chen J , et al.
    PubMed:10781902
    MGI:J:61814
    Exp Hematol 28(4):442-50

    2000
    Quantitative trait loci controlling allergen-induced airway hyperresponsiveness in inbred mice.
    Ewart SL , et al.
    PubMed:11017920
    MGI:J:66641
    Am J Respir Cell Mol Biol 23(4):537-45

    2000
    Differential maintenance and frequency-dependent tuning of LTP at hippocampal synapses of specific strains of inbred mice.
    Nguyen PV , et al.
    PubMed:11067991
    MGI:J:71278
    J Neurophysiol 84(5):2484-93

    2000
    Genetic heterogeneity of angiogenesis in mice.
    Rohan RM , et al.
    PubMed:10783140
    MGI:J:61808
    FASEB J 14(7):871-6

    2000
    The Dalila effect: C57BL6 mice barber whiskers by plucking.
    Sarna JR , et al.
    PubMed:10680755
    MGI:J:71538
    Behav Brain Res 108(1):39-45

    2000
    Macronutrient diet selection in thirteen mouse strains.
    Smith BK , et al.
    PubMed:10749765
    MGI:J:61602
    Am J Physiol Regul Integr Comp Physiol 278(4):R797-805

    1999
    Quantitative trait loci for bone density in C57BL/6J and CAST/EiJ inbred mice.
    Beamer WG , et al.
    PubMed:10556421
    MGI:J:58152
    Mamm Genome 10(11):1043-9

    1998
    Comparison of acute endotoxin-induced lesions in A/J and C57BL/6J mice.
    O'Malley J , et al.
    PubMed:9864862
    MGI:J:51631
    J Hered 89(6):525-30

    1998
    Reversal of diet-induced obesity and diabetes in C57BL/6J mice.
    Parekh PI , et al.
    PubMed:9751238
    MGI:J:49858
    Metabolism 47(9):1089-96

    1998
    Ltx1, a mouse locus that influences the susceptibility of macrophages to cytolysis caused by intoxication with Bacillus anthracis lethal factor, maps to chromosome 11.
    Roberts JE , et al.
    PubMed:9720874
    MGI:J:49726
    Mol Microbiol 29(2):581-91

    1997
    Distortion product otoacoustic emissions in the C57BL/6J mouse model of age-related hearing loss.
    Parham K , et al.
    PubMed:9367243
    MGI:J:44149
    Hear Res 112(1-2):216-34

    1996
    Genetic variability in adult bone density among inbred strains of mice.
    Beamer WG , et al.
    PubMed:8739896
    MGI:J:33789
    Bone 18(5):397-403

    1996
    Genetics of age-related hearing loss in mice. III. Susceptibility of inbred and F1 hybrid strains to noise-induced hearing loss.
    Erway LC , et al.
    PubMed:8735078
    MGI:J:33970
    Hear Res 93(1-2):181-7

    1995
    Hyper- and hypo-responsiveness to dietary fat and cholesterol among inbred mice: searching for level and variability genes.
    Kirk EA , et al.
    PubMed:7595076
    MGI:J:28648
    J Lipid Res 36(7):1522-32

    1995
    Genetics of responsiveness to high-fat and high- cholesterol diets in the mouse.
    Paigen B , et al.
    PubMed:7625360
    MGI:J:28248
    Am J Clin Nutr 62(2):458S-462S

    1994
    Molecular analysis of reverse mutations from nonagouti (a) to black-and-tan (a(t)) and white-bellied agouti (Aw) reveals alternative forms of agouti transcripts.
    Bultman SJ , et al.
    PubMed:8125260
    MGI:J:16984
    Genes Dev 8(4):481-90

    1994
    Microphthalmia and associated abnormalities in inbred black mice.
    Smith RS , et al.
    PubMed:7898027
    MGI:J:24131
    Lab Anim Sci 44(6):551-60

    1994
    Differences in dorsal and ventral pigmentation result from regional expression of the mouse agouti gene.
    Vrieling H , et al.
    PubMed:8202545
    MGI:J:18750
    Proc Natl Acad Sci U S A 91(12):5667-71

    1993
    Cloning of the mouse agouti gene predicts a secreted protein ubiquitously expressed in mice carrying the lethal yellow mutation.
    Miller MW , et al.
    PubMed:8449404
    MGI:J:4186
    Genes Dev 7(3):454-67

    1993
    Atherosclerosis and plasma and liver lipids in nine inbred strains of mice.
    Nishina PM , et al.
    PubMed:8355588
    MGI:J:13267
    Lipids 28(7):599-605

    1992
    Molecular characterization of the mouse agouti locus.
    Bultman SJ , et al.
    PubMed:1473152
    MGI:J:3523
    Cell 71(7):1195-204

    1992
    Dietary obesity in nine inbred mouse strains.
    West DB , et al.
    PubMed:1621856
    MGI:J:1348
    Am J Physiol 262(6 Pt 2):R1025-32

    1990
    Genetic heterogeneity of plasma lipoproteins in the mouse: control of low density lipoprotein particle sizes by genetic factors.
    Jiao S , et al.
    PubMed:1971301
    MGI:J:15484
    J Lipid Res 31(3):467-77

    1990
    Synthetic low and high fat diets for the study of atherosclerosis in the mouse.
    Nishina PM , et al.
    PubMed:2380634
    MGI:J:27046
    J Lipid Res 31(5):859-69

    1990
    Atherosclerosis susceptibility differences among progenitors of recombinant inbred strains of mice.
    Paigen B , et al.
    PubMed:2317166
    MGI:J:22615
    Arteriosclerosis 10(2):316-23

    1986
    Differences in susceptibility of inbred mice to Bacillus anthracis.
    Welkos SL , et al.
    PubMed:3081444
    MGI:J:8197
    Infect Immun 51(3):795-800

    1982
    Evidence for genetic control of microwave-induced augmentation of complement receptor-bearing B lymphocytes.
    Schlagel CJ , et al.
    PubMed:6980940
    MGI:J:6835
    J Immunol 129(4):1530-3

    1972
    Genetic relationships between inbred strains of mice.
    Taylor BA , et al.
    PubMed:5031317
    MGI:J:24288
    J Hered 63(2):83-6


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外观
被毛颜色
详情

Black

Related Genotype: a/a